The CHUM Research Centre offers a full range of multiparametric
cell analysis services, including high-throughput cell sorting in a
biosafety level 3 (BSL 3) containment suite that allows researchers
to sort cells from patients infected with HIV, HCV, or other
viruses.
This service is available to researchers from academic, hospital
and industrial settings.
Our services include:
- High speed cell sorting for the purification of discrete
primary cell subsets (peripheral blood or other tissues) or cell
lines.
- Multi-parametric analysis of cells and other particles:
simultaneous analysis of several functional parameters in
combination with surface markers. This allows identification and
analysis of complex systems such as new mechanisms of cell immunity
and viral pathogenesis.
Information:
Facility coordinators : cytometrie.crchum@ssss.gouv.qc.ca,
Laurence Lejeune, 514 890-8000 ext:
35726 or Annie Gosselin, 514 890-8000 ext: 34210
Scientific Director, Petronela Ancuta, petronela.ancuta@umontreal.ca,
514 890-8000 ext: 35744
Services
Equipment
Rates
Useful Links
SERVICES
Cell sorting – infectious and
non-infectious samples
Cell or particle analysis
Computer analysis of flow cytometry
data
Training
Development and technical validation
Cell sorting – infectious and non-infectious
samples
Two
BD ARIAII cell sorters, one of which is located in a BSL3
containment suite.
-
Simultaneous purification of up to 4 different
cell subsets, isolation of cell clones in 96-well plates, or cell
sorting directly on slides for various applications.
-
3 lasers (488nm, 633nm & 405nm) enable the
analysis of up to 10 different fluorochromes.
Our equipment is maintained under RNAse-free and sterile
conditions, which allows subsequent cell culture and
transcriptional studies.
Sorting is performed by an experienced technician.
Cell or particle analysis
Three flow cytometers are available for analysis of cells and
particles by size, granularity and emitted fluorescence.
Computer analysis of flow cytometry data
The cytometry service provides two computers and a variety of
software packages including Diva, Modfit and FCAP array for data
analysis.
Training
In addition to offering basic training for access to the
LSR II, we can also provide individualized training adapted to
specific research projects.
Development and technical validation
We can help you get your projects underway and develop
validation protocols adapted to your needs.
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